PML RARA FISH
Acute Promyelocytic Leukemia (APL); Acute Myeloid Leukemia (AML); Acute Myelogenous Leukemia
- Tech Only CPT
- Tech Pro CPT 88377
- PowerPath Code PMLRARA-FISH
- Schedule Daily
- Turn Around Time 24-48 hrs
- Methodology FISH
Organs
Specimen Requirements
Minimum 1mL of bone marrow aspirate or peripheral blood in green top sodium heparin (preferred) or purple top EDTA (acceptable). Contact FISH lab for fresh tissue or other fluid.
Diagnostic Utility
This gene rearrangement is a confirmatory testing for a diagnosis of APL.
Clinical Significance
In conjunction of clinical, morphologic, and cytogenetic studies, this rapid FISH testing plays a key role in diagnosis of APL. This FISH result also provides a targeted, effective treatment option. This assay can also be used for prognosis stratification, drug response and disease monitoring. The probe used for this assay is PML-RARA; t(15;17)(q24.1;q21)
Storage and Transportation
Room temperature, express mail if not on courier service
Cause for Rejection
Improperly labelled specimens will be rejected (must have two patient identifiers); frozen blood or bone marrow; FFPE blocks. PB and BM specimens collected in containers other than EDTA and NaHep; specimens >7 days old
Retention
5 days for original sample; 1 month for fixed cell pellets
Comments
Clinical indication and specimen source is required for the assay. Preliminary results available within 24 hours.