JAK2_V617F reflex to JAK2_Exon 12-16

Minimum of 1 mL whole blood in a purple (EDTA) or pink top tube (EDTA); minimum of 1 mL of bone marrow aspirate in a purple, pink, or green (heparin) top tube.

The V617F mutation in the Janus nonreceptor tyrosine kinase 2 (JAK2) gene has been reported in a significant proportion of patients with chronic myeloproliferative disorders (MPDs). The somatic mutation occurs in the transformed hematopoietic precursor and has been identified in 65-97% of polycythemia vera. JAK2_Ex12-16 will be reflexed if JAK2_V617F mutation is not detected.

Copy of the referring client's requisition form to accompany specimen.

Refrigerated: 5 days; ambient (room temperature): 3 days; specimen must be received by laboratory within 7-days of collection.

Frozen specimen; clotted or grossly hemolyzed specimens; serum; specimens without 2 patient identifiers; serum or plasma, aliquots (previously open containers); specimen that were received >7-days after collection.

Whole blood and bone marrow - 1 week.

43399-5; 72333-8;