JAK2_V617F reflex to CALR/MPL/JAK2_Exon 12-16
Janus Kinase 2, Exon 12-16, MPL, CALR
- Tech Only CPT
- Tech Pro CPT 81270, 81338, 81219, 81479
- PowerPath Code JAK2rfxC-M-Ex12
- Schedule Monday and Thursday
- Turn Around Time 14-21 days
- Disease State Myeloproliferative Neoplasms
- Methodology Real-Time PCR, reflex to Next Generation Sequencing
Organs
Specimen Requirements
Minimum of 1 mL whole blood in a purple (EDTA) or pink top tube (EDTA); minimum of 1 mL of bone marrow aspirate in a purple, pink, or green (heparin) top tube.
Clinical Significance
The V617F mutation in the Janus nonreceptor tyrosine kinase 2 (JAK2) gene has been reported in a significant proportion of patients with chronic myeloproliferative disorders (MPDs). The somatic mutation occurs in the transformed hematopoietic precursor and has been identified in 65-97% of polycythemia vera, 23-57% of essential thrombocythemia, and 35-57% of myelofibrosis specimens. CALR, MPL, or JAK2_Ex12-16 will be reflexed if JAK2_V617F mutation is not detected.
Required Patient Info
Copy of the referring client's requisition form to accompany specimen.
Storage and Transportation
Refrigerated: 5 days; ambient (room temperature): 3 days; specimen must be received by laboratory within 7-days of collection.
Cause for Rejection
Frozen specimen; clotted or grossly hemolyzed specimens; serum; specimens without 2 patient identifiers; serum or plasma, aliquots (previously open containers); specimen that were received >7-days after collection.
Retention
Whole blood and bone marrow - 1 week.
LOINC Code(s)
43399-5; 72333-8; 77174-1; 56142-3; 75033-1; 55300-8; 80186-0