HMB-45/Ki67

Formalin-fixed, paraffin embedded tissue block or cell block, or 1 unstained charged slide for each test requested plus 3-4 additional unstained slides cut at 4 microns. Adequate space on each slide for control tissue is required.

Human Melanoma Black-45 (HMB-45), a cytoplasmic marker is specific for melanocytic lesions and has particular uses in detecting the maturation pattern expressed by benign melanocytic naevi. ions. Ki67, a nuclear and cell proliferation marker shows positivity in less than 1% of cells in BN. Generally, any positivity is only shown in the superficial aspects of the dermis. Its pattern of expression highlights the presence or absence of maturation, showing reactivity in more than 10% of cells at the deep aspect of melanoma lesions.