BRAF (V600E, V600D, V600K) PCR

  • Tech Only CPT
  • Tech Pro CPT
  • PowerPath Code BRAF CRC (Colorectal), BRAF MELANOMA (Skin) BRAF other (Thyroid)
  • Schedule Tuesday, Thursday
  • Turn Around Time 4-7 Days
  • Disease State Colon cancer, Melanoma
  • Methodology PCR

Specimen Requirements

Tumor TissueFormalin-fixed, paraffin-embedded tissue blocks; fixed in 10% neutral-buffered formalin for at least 6 hours, and not more than 48 hours. Tissue should be prepared in sections between 4 and 6 microns thick.

Diagnostic Utility

Colorectal Carcinoma &Melanoma

Storage and Transportation

Room temperature or refrigerated. Ship in cooled container during summer months.

Cause for Rejection

Tissue not verified for the presence of tumor and specimens fixed in fixatives other than formalin are not acceptable. Samples <10% tumor burden will not be tested, as false negative results cannot be ruled out. Surgical pathology report should be included. Decalcification compromises the specimen quality but PCR is still attempted.


1 Year


Only tissue that is clearly invasive carcinoma (established by histopathologic criteria) should be tested. Selection of tissue for the PCR assay should be performed by a pathologist.