AML+MDS FISH Panel

Synonyms

Acute Myeloid Leukemia (AML); Acute Myelogenous Leukemia; Myelodysplastic Syndrome (MDS); Myelodysplasia; Bone Marrow Failure; Myelodysplatic Neoplasm

  • Tech Only CPT
  • Tech Pro CPT 88377x9
  • PowerPath Code AML+MDSFISH
  • Schedule Daily
  • Turn Around Time 2-3 days
  • Disease State Acute Myeloid Leukemia (AML) / Myeloid Sarcoma, Myeloid Sarcoma
  • Methodology FISH

Organs

Heme

Specimen Requirements

Minimum 1mL of bone marrow aspirate or peripheral blood in green top sodium heparin (preferred) or purple top EDTA (acceptable). Contact FISH lab for fresh tissue or other fluid.

Diagnostic Utility

The targeted gene rearrangements are AML defining abnormalities and can commonly be detected in adult patients with AML. The targeted gene gains or losses are the common chromosome abnormalities seen in patients with myelodysplastic syndromes (MDS), secondary AML, or other myeloid malignancies. This FISH assay is not validated for residual AML or MRD monitoring, especially on deletion or monosomy changes.

Clinical Significance

In conjunction with morphologic, cytogenetic, and molecular studies, the targeted chromosome abnormalities in this FISH panel play a key role in the evaluation of patients with MDS and AML, or other myeloid malignancies, and can be used in prognosis stratification, drug response, and disease monitoring.

AML+MDS FISH Panel includes the following probes:
RUNX1-RUNX1T1; t(8;21)(q21.3;q22.1)
KMT2A rearrangement; 11q23
PML-RARA; t(15;17)(q24.1;q21)
CBFB rearrangement; 16q22.1
Del(5q); 5p15.3/5q31.2
Monosomy 7/del(7q); CEP7/7q31.2
Trisomy 8; CEP8
TP53 deletion; 17p13/CEP17
Del(20q); 20q12/20q13.1

Storage and Transportation

Room temperature, express mail if not on courier service

Cause for Rejection

Improperly labelled specimens will be rejected (must have two patient identifiers); frozen blood or bone marrow; FFPE blocks. PB and BM specimens collected in containers other than EDTA and NaHep; specimens >7 days old

Retention

5 days for original sample; 1 month for fixed cell pellets

Comments

Clinical indication and specimen source is required for the assay.

Vendor

Cytocell