AML FISH Panel

Synonyms

Acute Myeloid Leukemia; Acute Myelogenous Leukemia

  • Tech Only CPT
  • Tech Pro CPT 88377x4
  • PowerPath Code AMLFISH
  • Schedule Daily
  • Turn Around Time 2-3 days
  • Disease State Acute Myeloid Leukemia (AML) / Myeloid Sarcoma
  • Methodology FISH

Organs

Heme

Specimen Requirements

Minimum 1mL of bone marrow aspirate or peripheral blood in green top sodium heparin (preferred) or purple top EDTA (acceptable). Contact FISH lab for fresh tissue or other fluid.

Diagnostic Utility

The targeted gene rearrangements are AML defining abnormalities and can commonly be detected in adult patients with AML. This FISH assay is not validated for residual AML or MRD monitoring.

Clinical Significance

The AML FISH panel is useful at initial diagnosis for detecting recurrent common chromosome abnormalities in adult patients with AML. These chromosome alterations can also be used for prognosis stratification, drug response and disease monitoring.

AML FISH Panel includes the following probes:

  • RUNX1-RUNX1T1; t(8;21)(q21.3;q22.1)
  • KMT2A; 11q23
  • PML-RARA; t(15;17)(q24.1;q21)
  • CBFB; 16q22.1

Storage and Transportation

Room temperature, express mail if not on courier service

Cause for Rejection

Improperly labelled specimens will be rejected (must have two patient identifiers); frozen blood or bone marrow; FFPE blocks. PB and BM specimens collected in containers other than EDTA and NaHep; specimens >7 days old

Retention

5 days for original sample; 1 month for fixed cell pellets

Comments

Clinical indication and specimen source is required for the assay.

Vendor

Cytocell