TP53 NGS

Minimum of 1 mL whole blood in a purple (EDTA) or pink top tube (EDTA); minimum of 1 mL of bone marrow aspirate in a purple, pink, or green (heparin) top tube. Fresh tissue in saline or RPMI, FFPE blocks.

TP53 mutations have been detected in about 15% to 20% of patients with mantle cell lymphoma (MCL) and in about 5% to 12% of patients with chronic lymphocytic leukemia (CLL). MCL or CLL harboring TP53 mutations have been associated with an adverse prognosis. This sequencing analysis is designed to detect small mutations (including single nucleotide variations (SNV) and small insertions or deletions) within the coding exons and splicing sites of the TP53 gene, but not to detect the entire gene deletion at 17p13 locus. Fluorescent in situ hybridization (FISH) is the current method for 17p13 analysis.

Copy of the referring client's requisition form to accompany specimen.

Refrigerated: 5 days; ambient (room temperature): 3 days; specimen must be received by laboratory within 7 days of collection.

Frozen specimen; clotted or grossly hemolyzed specimens; serum; specimens without 2 patient identifiers; serum or plasma, aliquots (previously open containers); specimen that were received >7 days after collection.

Whole blood and bone marrow - 1 week.