Bone Marrow Aspiration and Biopsy

  • Tech Only CPT
  • Tech Pro CPT
  • PowerPath Code
  • Schedule Monday - Friday (Sat. by arrangement)
  • Turn Around Time 1-2 days
  • Disease State Leukemia, Lymphoma
  • Methodology Tissue

Specimen Requirements

It is recommended that specimens be received within 24 hours of collection
1. Bone marrow biopsy: Submit two (2) air-dried imprint smears from fresh biopsy core specimen. Submit one 1 cm core bone marrow in 10% neutral buffered formalin.
2. Bone marrow smears: Submit six (6) air-dried smears in slide holders. Ensure the slides are thoroughly dried before closing the slide holder to prevent specimen sticking to the slide holder.
3. Peripheral smears: Submit two (2) air-dried smears in slide holders. Ensure the slides are thoroughly dried before closing the slide holder to prevent destruction of the specimen.
4. Bone marrow clot: Collect the 1-5 mL bone marrow aspirate. Allow the specimen to clot for 4-5 minutes in the syringe. Submit the clotted 1-5 mL bone marrow aspirate in 10% neutral buffered formalin.
5. All slides and containers must be labeled with patient’s full first and last name, date of birth and source/site.
6. All specimens should be submitted on one requisition.

Required Patient Info

All specimens and slides should be labeled with two patient identifiers.
Must include patient history and suspected diagnosis.
Should include any appropriate clinical results (e.g. CBC, WBC, etc…).
Send a completed requisition. If not initially ordered, please indicate if considering adding molecular testing orders so bone processing will not include decalcification.

Storage and Transportation

Maintain at room temperature
Bone marrow kits or other collection supplies are available from CellNetix through the LabDox portal, on our Supplies page or by calling the Seattle based support team at 844-344-4209.
Specimens should be shipped to CellNetix in the provided FedEx packaging. Always include an added ice pack but be sure that it is not in direct contact with the tissue.
Note that specimens shipped on a Friday will not be delivered on Saturday unless you alert the shipping vendor ahead of time.

Cause for Rejection

If insufficient cellular material is present, the technologist/pathologist may decide the material is insufficient for proper evaluation. · Unsatisfactory specimens include acellular specimens, severe obscuring inflammation or blood, extensive air-drying artifact, or extensive mechanical distortion. · Specimen containers without two (2) patient identifiers.

Retention

Fresh specimen – 2 weeksSlide(s) and paraffin block(s) – 10 years

Comments

Include pertinent clinical information, e.g. previous malignancy, drugs, radiation therapy, and other history. The most recent CBC report should be submitted with the specimen.

Note that specimens shipped on a Friday will not be delivered on Saturday unless you alert the shipping vendor ahead of time. A routine specimen that arrives before 3:00 pm will be processed and available for the pathologist to review no later than the next business day. Most cases that do not require additional special testing will be signed out within 3 business days.

Expedited handling of late-arriving samples including weekend or same-day urgent handling are available on a case-by-case basis by prior arrangement. Please call support at 844-344-4209 and ask for the hematopathologist on call.

Specialized testing including cytogenetics or next-generation sequencing will usually be resulted in an addendum within two to four weeks of the initial diagnosis.