Calreticulin

Synonyms

CALR,CALR_Exon 9 (insertion or deletion)

  • Tech Only CPT
  • Tech Pro CPT 81219, G0452
  • PowerPath Code CALR ex9in
  • Schedule Monday to Friday
  • Turn Around Time 4-6 days
  • Disease State Myeloproliferative Neoplasms
  • Methodology PCR

Specimen Requirements

Container Type: Lavender top tube (EDTA)Specimen Type: Whole Blood; Bone MarrowPreferred Volume: 5mL Whole Blood;2 mL Bone MarrowMinimum Volume:3 mL Whole Blood; 1 mL Bone Marrow

Clinical Signifigance

Somatic mutations in CALR gene, which encodes endoplasmic reticulum chaperone Calreticulin, were found in majority of patients with Myeloproliferative neoplasms without JAK2 mutation. Insertions or deletions in exon 9 of CALR were detected in 67% of patients with essential thrombocythemia (ET) and 88% of those with primary myelofibrosis (PMF), but rarely detected in patients with polycythemia vera. This assay was designed to detect all types of insertions and deletions in exon 9 of CALR that has been reported. .

Required Patient Info

Copy of the referring clinet's requisition form to accompany specimen.

Storage and Transportation

Refrigerated:5 daysAmbient (room temperature): 3 days

Cause for Rejection

Frozen specimen; clotted or grossly hemolyzed specimens; serum; specimens without 2 patient identifiers; serum or plasma, aliquots (previously open containers).

Retention

one week - whole blood. DNA - 2 years.