Bone Marrow Aspiration and Biopsy

  • Tech Only CPT
  • Tech Pro CPT 85097, 88305, 88311. 88313
  • PowerPath Code
  • Schedule Monday - Friday (Sat. by arrangement)
  • Turn Around Time 1-2 days
  • Disease State Leukemia, Lymphoma
  • Methodology Tissue

Specimen Requirements

It is recommended that specimens be received within 24 hours of collection1. Bone marrow biopsy: Submit two (2) air-dried imprint smears from fresh biopsy core specimen. Submit one 1 cm core bone marrow in 10% neutral buffered formalin.2. Bone marrow smears: Submit six (6) air-dried smears in slide holders. Ensure the slides are thoroughly dried before closing the slide holder to prevent specimen sticking to the slide holder.3. Peripheral smears: Submit two (2) air-dried smears in slide holders. Ensure the slides are thoroughly dried before closing the slide holder to prevent destruction of the specimen.4. Bone marrow clot: Collect the 1-5 mL bone marrow aspirate. Allow the specimen to clot for 4-5 minutes in the syringe. Submit the clotted 1-5 mL bone marrow aspirate in 10% neutral buffered formalin.5. All slides and containers must be labeled with patient’s full first and last name, date of birth and source/site.6. All specimens should be submitted on one requisition.

Storage and Transportation

Maintain at room temperature

Cause for Rejection

If insufficient cellular material is present, the technologist/pathologist may decide the material is insufficient for proper evaluation. · Unsatisfactory specimens include acellular specimens, severe obscuring inflammation or blood, extensive air-drying artifact, or extensive mechanical distortion. · Specimen containers without two (2) patient identifiers.

Retention

Fresh specimen – 2 weeksSlide(s) and paraffin block(s) – 10 years

Comments

Include pertinent clinical information, e.g. previous malignancy, drugs, radiation therapy, and other history. · The most recent CBC report should be submitted with the specimen.